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Mycolactone is cytotoxic for keratinocytes.

By Alvar Grönberg (360022), Louise Zettergren (360024), Kerstin Bergh (360026), Mona Ståhle (31955), Johan Heilborn (360028), Kristian Ängeby (317588), Pamela L. Small (360029), Hannah Akuffo (240013) and Sven Britton (240011)


<p>Mycolactone was added to sub-confluent cultures of keratinocytes and (A) cell numbers were measured as optical density of WST-1after 24, 48 and 72 h of treatment with 1000 ng/ml of mycolactone, or (B) after 72 h at different concentrations of mycolactone. An asterisk indicates a significant (p<0.05) reduction in WST-1 labeling as compared to the corresponding medium control. Cell cultures of untreated controls (C) and cultures treated with 300 ng/m mycolactone (D) were photographed after 48 h. Data are from one representative experiment showing means and SD of triplicates.</p

Topics: Cell Biology, cytotoxic
Year: 2013
DOI identifier: 10.1371/journal.pone.0013839.g001
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Provided by: FigShare
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