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TGF-β3 mRNA is upregulated in QNR/v-src<sup>ts</sup>/ICN cells.

By Samira Amarir (360555), Maria Marx (360557) and Georges Calothy (360558)

Abstract

<p>QNR/v-src<sup>ts</sup> and QNR/v-src<sup>ts</sup>/ICN cells were incubated 72 hrs at 37°C or 41°C before extraction of total RNA. After reverse transcription of 1 µg of RNA, TGF-β3 cDNA was amplified by QPCR using specific primers (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0013572#pone-0013572-t001" target="_blank">Table 1</a>) Results were normalized based on HPRT and TBP transcript levels and presented in relative arbitrary units. Normalized TGF-β3 mRNA levels detected in QNR/v-src<sup>ts</sup> cells at 37°C were used as reference equal to 1. Each bar represents the mean -/+ S.E. of experiments realized on 3 different cultures per cell type.</p

Topics: Cell Biology, Cancer, mrna, upregulated
Year: 2013
DOI identifier: 10.1371/journal.pone.0013572.g001
OAI identifier: oai:figshare.com:article/493311
Provided by: FigShare
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