PtdIns5<i>P</i> levels and ATX1 activity in <i>AtMTM1</i> mutant cells.

Abstract

<p>PtdIns5<i>P</i> levels in fresh and dehydrated wild type leaves, in OX-AtMTM1 leaves, and in leaves from homozygous <i>mtm1</i> plants (panel a). Relative <i>WRKY70</i> transcripts in non-stressed and in stressed wild-type, OX-AtMTM1, and <i>mtm1</i> mutant cells, quantified by real-time PCR (panel b). Quantitative PCR of ChIP assays of H3K4me3 methylation in wild-type, <i>OX-ATMTM1</i> and <i>mtm1</i> mutant chromatins in fresh leaves and in leaves after dehydration-stress (panel c). Relative enrichment in <i>WRKY70</i>-specific fragments <i>vs</i> input DNA.</p