Detergent screening of mAQP4 M23 expressed in the D-CF mode.

Lei Kai (361338); Ralf Kaldenhoff (16093); Jiazhang Lian (361339); Xiangcheng Zhu (361340); Volker Dötsch (361341); Frank Bernhard (361342); Peilin Cen (361343); Zhinan Xu (267082)

doi:10.1371/journal.pone.0012972.g002

Detergent screening of mAQP4 M23 expressed in the D-CF mode.

By Lei Kai (361338), Ralf Kaldenhoff (16093), Jiazhang Lian (361339), Xiangcheng Zhu (361340), Volker Dötsch (361341), Frank Bernhard (361342), Peilin Cen (361343) and Zhinan Xu (267082)

Abstract

<p>A: RM samples of 2 µl were analyzed by 16% SDS-PAGE and immunoblotted using anti-His antibodies. B: Solubility of D-CF expressed mAQP4 M23 in presence of 0.2% Brij-35, 0.4% Digitonin, 0.1% Triton X-100, and 0.05% Tyloxapol. Control is P-CF expressed mAQP4 M23. S, supernatant; P, pellet.</p

Topics: Biochemistry, Biotechnology, maqp4, m23, d-cf

Year: 2013

DOI identifier: 10.1371/journal.pone.0012972.g002

OAI identifier: oai:figshare.com:article/497653

Provided by: FigShare

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Detergent screening of mAQP4 M23 expressed in the D-CF mode.

Abstract

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