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RCAN1.4 induction requires Ca<sup>2+</sup>, calcineurin and PKC activity.

By Katherine Holmes (363469), Elinor Chapman (363470), Violaine See (5653477) and Michael J. Cross (363471)

Abstract

<p>HDMEC were incubated with the inhibitors ZM (3 µM), BAP (20 mM), EGTA (2.5 mM), GFX (3 µM), Go (3 µM). CsA (100 nM) or U0126 (5 µM) for 30 minutes, and then stimulated with: (<i>A</i>) VEGF-A (50 ng/ml), (<i>B</i>) PMA (100 nM), or (<i>C</i>) A23187 (5 µM) for 1 hour. Expression of <i>RCAN1.4</i> mRNA, relative to unstimulated (basal) cells, was determined by RT-qPCR. Results were normalised to <i>β-actin</i> expression. Data shows mean ± SD (n = 3). **P<0.01, versus VEGF/PMA/A23187 treated. BAP – BAPTA/AM, CsA – cyclosporin A, GFX - GF109203X, Go – Gö6976, ZM – ZM323881.</p

Topics: Medicine, Cell Biology, induction, requires, calcineurin, pkc
Year: 2013
DOI identifier: 10.1371/journal.pone.0011435.g004
OAI identifier: oai:figshare.com:article/512033
Provided by: FigShare
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