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Functional and physical link between CD9P-1 and α2β1 integrin.

By Célia Chambrion (363962) and François Le Naour (43849)


<p>(A) Time-lapse videomicroscopy experiments were performed with HEK-293 or HEK/CD9P-1 cells. Monoclonal antibodies directed against integrins α1 (FB12) or α2 (Gi9) were added in the media (10 µg/ml). Independent experiments were performed (n = 6); standard errors are shown; (*) p<0.01. (B) Cells were lysed using the mild detergent Brij58 and immunoprecipitation experiments were performed with mAbs directed against CD9P-1 (1F11), α2-integrin (Gi9), β1-integrin (9D6) and CD55. After electrophoresis and transfer to a membrane, Western blotting was performed using α2-integrin antibody H-293.</p

Topics: Cell Biology, cd9p-1
Year: 2013
DOI identifier: 10.1371/journal.pone.0011219.g004
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Provided by: FigShare
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