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Fluorescent micrograph of Mtb detected by IF and AR in mouse and guinea pig.

By Gavin J. Ryan (354468), Donald R. Hoff (354466), Emily R. Driver (354469), Martin I. Voskuil (44321), Mercedes Gonzalez-Juarrero (364123), Randall J. Basaraba (129586), Dean C. Crick (364125), John S. Spencer (168814) and Anne J. Lenaerts (354472)

Abstract

<p>Mtb was detected by IF (A,D,G,J), AR (B,E,H) and combined IF-AR (C,F,I) in C57BL/6 mouse lung (A–C), GKO mouse lung (D–F) and guinea pig lung (G–I). 5 µm formalin fixed paraffin embedded sections were dewaxed, rehydrated and detected by IF. Rabbit polyclonal antibody against whole Mtb cell lysate was applied overnight at 4°C and detected using an Alexafluor 488 labeled secondary antibody (green). Slides were then stained with AR (orange/red) for 30 mins then destained. Pictures were taken using a FITC and TRITC filter at 400× magnification. (J) Confocal micrograph of Mtb detected by IF in GKO lung tissue at 1000× magnification with 1.6× digital zoom.</p

Topics: Microbiology, Cell Biology, Infectious Diseases, micrograph, mtb, ar, guinea
Year: 2013
DOI identifier: 10.1371/journal.pone.0011108.g003
OAI identifier: oai:figshare.com:article/515904
Provided by: FigShare
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