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Increased apoptosis in AM with deficient CFTR.

By Yaqin Xu (364234), Anja Krause (364235), Hiroko Hamai (364236), Ben-Gary Harvey (255775), Tilla S. Worgall (364237) and Stefan Worgall (364238)

Abstract

<p>AM transfected with CFTR-siRNA or control-siRNA for 48 h were analyzed for apoptosis by TUNEL assay and for cleaved PARP protein expression by Western analysis. <b>A.</b> TUNEL assay. The nuclear staining of green fluorescence was shown as the positive apoptosis signal. DAPI served as normal nuclear staining control. <b>B.</b> Quantification of TUNEL assay. <b>C.</b> IL-8 secretion adjusted to the percentage of non-apoptotic cells in AM transfected with CFTR-siRNA or control-siRNA after 48 h. <b>D.</b> Western analysis of cleaved PARP protein expression using β-tubulin as loading control. <b>E.</b> Quantification of Western analysis. Shown is the mean ± SEM of three pairs of independent samples. This experiment is the representative of 6 studies.</p

Topics: Medicine, Immunology, apoptosis, am, deficient
Year: 2013
DOI identifier: 10.1371/journal.pone.0011004.g003
OAI identifier: oai:figshare.com:article/516566
Provided by: FigShare
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