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Diagram of the lentivirus vector shuttle plasmids pLV.pA+.GS.DsRed and pLV.pA+.GS.Luc.

By Manuel A. F. V. Gonçalves (53335), Josephine M. Janssen (364316), Maarten Holkers (53336) and Antoine A. F. de Vries (53340)

Abstract

<p>Grey box with broken arrow, 5′ hybrid long terminal repeat (LTR) containing Rous sarcoma virus and HIV-1 sequences; Grey box without broken arrow, 3′ SIN LTR; ψ, HIV-1 packaging signal; Cyan arrowheads, FLP recognition target (FRT) sites; orange box with broken arrow, human <i>glyceraldehyde-3-phosphate dehydrogenase</i> (hGAPDH) gene promoter; Large and small green boxes, rabbit <i>β-globin</i> and murine <i>metallothionine</i> gene pAs (rBGpA and mMTpA, respectively); red box, <i>DsRed.T4-N1</i> ORF from <i>Discosoma sp</i>. (DsRed); yellow box, <i>luciferase</i> ORF from <i>Photinus pyralis</i> (PpLuc). All genetic elements are drawn to scale.</p

Topics: Medicine, Microbiology, Cell Biology, Genetics, Molecular Biology, Biotechnology, Virology, lentivirus, vector, plasmids
Year: 2013
DOI identifier: 10.1371/journal.pone.0010954.g002
OAI identifier: oai:figshare.com:article/516807
Provided by: FigShare
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