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PD153035 does not change mitochondrial respiratory parameters.

By Renata A. Cavalheiro (364722), Rodrigo M. Marin (243213), Silvana A. Rocco (255333), Fernanda M. Cerqueira (333810), Camille C. Caldeira da Silva (364723), Roberto Rittner (364724), Alicia J. Kowaltowski (284269), Anibal E. Vercesi (364725), Kleber G. Franchini (255354) and Roger F. Castilho (364726)

Abstract

<p>Rat heart mitochondria were incubated in 125 mM sucrose, 65 mM KCl, 10 mM HEPES, 2.5 mM KH<sub>2</sub>PO<sub>4</sub>, 0.4 mM EGTA, 1.25 mM malate, 1.25 mM glutamate, 1.25 mM pyruvate and 1.25 mM α-ketoglutarate, pH 7.2 (KOH), in the presence of 10 nM PD153035 or the equivalent concentration of PD153035 solvent DMSO (0.1%) (Control), as indicated. ADP (250 µM) and 1 µg/mL oligomycin were added to achieve state 3 and 4 respiratory rates, respectively. Respiration was measured using a Clark-type electrode, and respiratory parameters were calculated as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0010666#s2" target="_blank">Materials and Methods</a>. None of the values obtained in the presence of PD153035 are significantly different from controls (four independent preparations, experiments performed in triplicate).</p

Topics: Medicine, Cell Biology, mitochondrial, respiratory
Year: 2013
DOI identifier: 10.1371/journal.pone.0010666.t001
OAI identifier: oai:figshare.com:article/520010
Provided by: FigShare
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