<p>Rat heart mitochondria were incubated in 125 mM sucrose, 65 mM KCl, 10 mM HEPES, 2.5 mM KH<sub>2</sub>PO<sub>4</sub>, 0.4 mM EGTA, 1.25 mM malate, 1.25 mM glutamate, 1.25 mM pyruvate and 1.25 mM α-ketoglutarate, pH 7.2 (KOH), in the presence of 10 nM PD153035 or the equivalent concentration of PD153035 solvent DMSO (0.1%) (Control), as indicated. ADP (250 µM) and 1 µg/mL oligomycin were added to achieve state 3 and 4 respiratory rates, respectively. Respiration was measured using a Clark-type electrode, and respiratory parameters were calculated as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0010666#s2" target="_blank">Materials and Methods</a>. None of the values obtained in the presence of PD153035 are significantly different from controls (four independent preparations, experiments performed in triplicate).</p
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