siRNA against CCT-eta decreases EGF - induced fibroblast migration, whereas siRNA against CCT-beta does not.

Abstract

<p>Cells were incubated in the presence or absence of EGF(1 nM) +/− siRNA against CCT-eta (a) or CCT-beta (b) in an <i>in vitro</i> wound healing assay. In all experiments a subunit -specific scrambled siRNA sequence was used as control. Cell motility is displayed as a relative percentage of baseline motility in the absence of EGF or siRNA exposure (100%). Active siRNA versus CCT-eta reduced both basal and EGF-induced motility; siRNA versus CCT-beta and scrambled controls had no effect. Values are means ± SEM of six independent studies, each performed in triplicate. Statistical analyses were performed with Student's <i>t</i> test.</p

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