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siRNA against CCT-eta decreases EGF - induced fibroblast migration, whereas siRNA against CCT-beta does not.

By Latha Satish (365152), Sandra Johnson (365153), James H-C. Wang (365154), J. Christopher Post (60144), Garth D. Ehrlich (60143) and Sandeep Kathju (365155)

Abstract

<p>Cells were incubated in the presence or absence of EGF(1 nM) +/− siRNA against CCT-eta (a) or CCT-beta (b) in an <i>in vitro</i> wound healing assay. In all experiments a subunit -specific scrambled siRNA sequence was used as control. Cell motility is displayed as a relative percentage of baseline motility in the absence of EGF or siRNA exposure (100%). Active siRNA versus CCT-eta reduced both basal and EGF-induced motility; siRNA versus CCT-beta and scrambled controls had no effect. Values are means ± SEM of six independent studies, each performed in triplicate. Statistical analyses were performed with Student's <i>t</i> test.</p

Topics: Cell Biology, cct-eta, decreases, egf, induced, fibroblast, sirna, cct-beta
Year: 2013
DOI identifier: 10.1371/journal.pone.0010063.g004
OAI identifier: oai:figshare.com:article/522211
Provided by: FigShare
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