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siRNA against CCT-eta decreases PDGF-induced fibroblast migration, whereas siRNA against CCT-beta does not.

By Latha Satish (365152), Sandra Johnson (365153), James H-C. Wang (365154), J. Christopher Post (60144), Garth D. Ehrlich (60143) and Sandeep Kathju (365155)

Abstract

<p>Cells were incubated in the presence or absence of PDGF (200 nM) +/− siRNA against CCT-eta (a) or CCT-beta (b) in an <i>in vitro</i> wound healing assay. In all experiments a subunit -specific scrambled siRNA sequence was used as control. Cell motility is shown as a percentage of baseline migration in the absence of PDGF or siRNA exposure. As with EGF, active siRNA targeting CCT-eta inhibited basal and PDGF-induced motility, whereas CCT-beta siRNA and scrambled controls did not. Values are means ± SEM of six independent studies, each performed in triplicate. Statistical analyses were performed with Student's <i>t</i> test.</p

Topics: Cell Biology, cct-eta, decreases, pdgf-induced, fibroblast, sirna, cct-beta
Year: 2013
DOI identifier: 10.1371/journal.pone.0010063.g005
OAI identifier: oai:figshare.com:article/522296
Provided by: FigShare
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