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siRNA against α-SMA inhibits both basal and EGF-induced cell migration in adult fibroblasts.

By Latha Satish (365152), Sandra Johnson (365153), James H-C. Wang (365154), J. Christopher Post (60144), Garth D. Ehrlich (60143) and Sandeep Kathju (365155)


<p>Cells were incubated in the presence or absence of EGF(1 nM) +/− siRNA against α-SMA in an <i>in vitro</i> wound healing assay. In all experiments a non-specific control siRNA was used as a control. Cell motility is displayed as a relative percentage of baseline motility in the absence of EGF or siRNA exposure (100%). Active siRNA versus α-SMA reduced both basal and EGF-induced motility; a non-specific control siRNA had no such effect. Values are means ± SEM of eight independent studies, each performed in duplicate. Statistical analyses were performed with Student's <i>t</i> test.</p

Topics: Cell Biology, inhibits, basal, egf-induced
Year: 2013
DOI identifier: 10.1371/journal.pone.0010063.g011
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Provided by: FigShare
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