Article thumbnail

siRNA against α-SMA inhibits both basal and EGF-induced cell migration in adult fibroblasts.

By Latha Satish (365152), Sandra Johnson (365153), James H-C. Wang (365154), J. Christopher Post (60144), Garth D. Ehrlich (60143) and Sandeep Kathju (365155)

Abstract

<p>Cells were incubated in the presence or absence of EGF(1 nM) +/− siRNA against α-SMA in an <i>in vitro</i> wound healing assay. In all experiments a non-specific control siRNA was used as a control. Cell motility is displayed as a relative percentage of baseline motility in the absence of EGF or siRNA exposure (100%). Active siRNA versus α-SMA reduced both basal and EGF-induced motility; a non-specific control siRNA had no such effect. Values are means ± SEM of eight independent studies, each performed in duplicate. Statistical analyses were performed with Student's <i>t</i> test.</p

Topics: Cell Biology, inhibits, basal, egf-induced
Year: 2013
DOI identifier: 10.1371/journal.pone.0010063.g011
OAI identifier: oai:figshare.com:article/522825
Provided by: FigShare
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • https://figshare.com/articles/... (external link)
  • Suggested articles


    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.