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A novel approach to investigating the erythroid lineage, using both receptor analysis and haemoglobin detection

By Colin P. McGuckin, Wai M. Liu, Edward C. Gordon-Smith and Mario R. Uhr

Abstract

Progenitor cell failure in the erythroid lineage is a particular problem in bone marrow failure. To provide insight into early erythopoietic development we used sensitive techniques to examine the effects of SCF, IL-3 and MIP-1 alpha on two developmentally arrested progenitor cell lines, HEL and K562. Quantitative flowcytometric analysis showed that both expressed receptors (SCF > MIP-1 alpha > IL-3). Qualitative analysis revealed HEL cells expressed more receptors than K562 cells. Clonogenic assays with sensitive haemoglobin detection showed that SCF and IL-3 did not support HEL development and reduced haemoglobin production. MIP-1 alpha reduced partially developed HEL colonies and haemoglobin in developed colonies. SCF increased development, but not haemoglobin in K562 cells, with IL-3 being more effective in both. MIP-1 alpha increased the proportion of well-developed K562 colonies but not haemoglobin. This suggests SCF, IL-3 and MIP-1 alpha all have a role to play in early erythroid cellular development, with differing actions depending on the stage of development

Topics: alliedhealth
Publisher: Blackwell Publishing
Year: 1996
DOI identifier: 10.1046/j.1365-2141.1996.d01-1942.x
OAI identifier: oai:eprints.kingston.ac.uk:2491
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