Article thumbnail

PBMCs stimulated with cross-reactive microbial peptides can suppress HIV-1 infection.

By Christopher W. Pohlmeyer (3142515), Sarah B. Laskey (3142518), Sarah E. Beck (4873063), Daniel C. Xu (4873072), Adam A. Capoferri (4873078), Caroline C. Garliss (4873054), Megan E. May (4873051), Alison Livingston (4873069), Walt Lichmira (4873075), Richard D. Moore (340677), M. Sue Leffell (4873057), Nicholas J. Butler (4873066), Jennifer E. Thorne (486304), John A. Flynn (4873060), Robert F. Siliciano (148667) and Joel N. Blankson (148662)

Abstract

<p>(<b>A-B</b>) Results of PBMC suppression assay. PBMCs from HIV-1-positive HLA-B*27<sup>+</sup> subjects were stimulated for five days with no peptide, CEF pooled peptides, KK10 peptide, or KK10CR peptide and then infected with highly concentrated IIIB virus. After 40 hours, infection of CD3<sup>+</sup>/CD8<sup>-</sup> cells was measured by staining for intracellular HIV-1 Gag. Values below 20% (in grey) fall below the limit of quantification. Part <b>A</b> shows flow cytometric results for subject ES31; part <b>B</b> shows summarized results for five subjects with CEF pooled peptides, KK10 peptide, and individual KK10CR peptides (<b>C-D</b>) PBMCs from HIV-1-positive HLA-A2<sup>+</sup> subjects were stimulated for five days with no peptide, CEF pooled peptides, SL9 peptide, or SL9CR peptide and then infected with highly concentrated IIIB virus. After 40 hours, infection of CD3<sup>+</sup>/CD8<sup>-</sup> cells was measured by staining for intracellular HIV-1 Gag. Part <b>C</b> shows flow cytometric results for subject CP37; part <b>D</b> shows summarized results for seven subjects with CEF pooled peptides, SL9 peptide, and individual SL9CR peptides.</p

Topics: Microbiology, Cell Biology, Immunology, Cancer, Infectious Diseases, Virology, Biological Sciences not elsewhere classified, PBMC, HIV-specific CD 8, TCR, HIV -1-specific immunity, HIV -1-specific CD 8, T cell response, peptide, T cells, HIV -1-positive subjects, cross-reactive, autologous CD 4, T cell responses Heterologous immunity
Year: 2018
DOI identifier: 10.1371/journal.pone.0192098.g004
OAI identifier: oai:figshare.com:article/5912899
Provided by: FigShare
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • https://figshare.com/articles/... (external link)
  • Suggested articles


    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.