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Lymphocyte changes in LN after hetIL-15 treatment.

By Dionysios C. Watson (4885495), Eirini Moysi (152053), Antonio Valentin (396219), Cristina Bergamaschi (4885504), Santhi Devasundaram (4885501), Sotirios P. Fortis (4885498), Jenifer Bear (534909), Elena Chertova (375858), Julian Bess Jr. (4885510), Ray Sowder (4885507), David J. Venzon (50683), Claire Deleage (322572), Jacob D. Estes (194109), Jeffrey D. Lifson (140183), Constantinos Petrovas (517531), Barbara K. Felber (189501) and George N. Pavlakis (45947)

Abstract

<p>(A) Step-dose regimen of six SC hetIL-15 administrations in rhesus macaques. LN, blood and mucosal tissue lymphocytes were analyzed before (pre) and after treatment (+hetIL-15). Flow cytometry dot plots of LN mononuclear cells show (B) the frequency of CD8<sup>+</sup> memory subsets, naïve (T<sub>N</sub>, CD28<sup>+</sup>CD95<sup>low</sup>), central memory (T<sub>CM</sub>, CD28<sup>high</sup>CD95<sup>+</sup>) and effector memory (T<sub>EM</sub>, CD28<sup>-</sup>CD95<sup>+</sup>), and (D) granzyme B content and cycling status (GrzB<sup>+</sup>Ki67<sup>+</sup>) from a representative uninfected macaque (R921) upon hetIL-15 treatment. Graphs (C, E, F) summarize results of 16 macaques treated with hetIL-15 of (C) frequency of effector memory CD8<sup>+</sup> T cells, (E) CD8<sup>+</sup>GrzB<sup>+</sup> T cells, and (F) cycling (Ki67+) CD8<sup>+</sup> T cells. Analysis was performed on LN of 9 uninfected animals (filled symbols) and 7 SHIV<sup>+</sup> macaques (open symbols). Black symbols, pre; red symbols, +hetIL-15. P values are from paired Wilcoxon signed rank test. The 12 animals that were also analyzed for hetIL-15 effects in blood and mucosal tissues (Figs <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1006902#ppat.1006902.g002" target="_blank">2</a> and <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1006902#ppat.1006902.g003" target="_blank">3</a>) are indicated by *.</p

Topics: Biochemistry, Medicine, Cell Biology, Molecular Biology, Physiology, Immunology, Cancer, Infectious Diseases, Virology, Chemical Sciences not elsewhere classified, lymph nodes B cell follicles, heterodimeric IL -15 increases cytotoxic lymphocytes, SHIV RNA, hetIL -15 treatment, effector CD 8, cytotoxic CD 8, FH, B cell follicles, hetIL -15, virus-specific MHC-peptide tetramer, combination immunotherapy regimens, LN, heterodimeric IL -15, T cells, NCT, target AIDS virus sanctuaries, granzyme B content
Year: 2018
DOI identifier: 10.1371/journal.ppat.1006902.g001
OAI identifier: oai:figshare.com:article/5922982
Provided by: FigShare
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