Localization of LsVg and RSV in female <i>L</i>. <i>striatellus</i> tissues.
<p>RSV was probed with Alexa Fluor 488-labeled mouse anti-RSV monoclonal antibody (shown in green). LsVg was probed with mouse anti-LsVg monoclonal antibody Ab47Km and stained with Alexa Fluor 568 (shown in red). Nucleoli were stained with TO-PRO-3 (shown in blue). Images were examined using a Leica TCS SP8 confocal microscope. Images are representative of three independent experiments with a total of 15 SBPHs analyzed. The white arrow indicates the immune-reactive signal of the LsVg protein. The scale bar represents 20 μm.</p
Biochemistry, Medicine, Microbiology, Molecular Biology, Physiology, Evolutionary Biology, Ecology, Infectious Diseases, Plant Biology, Virology, Computational Biology, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, body-produced LsVg, RSV titer, striatellus individuals, plant virus Insect vitellogenin, Vg protein, Insect tissue-specific vitellogenin, RNA interference, tissue-specific processing, virus transmission, LsVg subunit, virus infection, subunit composition, LsVg transcripts, Rice stripe virus, proteins co-localize, hemocyte-produced Vg, striatellus Vg, RSV-interacting subunit, sex-independent expression, Laodelphax striatellus hemocytes
DOI identifier: 10.1371/journal.ppat.1006909.g002
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