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Study on Preparation of Functional Polymer Microspheres arid. Application in certain fields

By 徐世义


功能高分子微球由于具有粒径小、比表面积大以及含有多种功能基团,在诸如生物化学及生物医学、微电子工业等领域具有广泛的应用前景。由于其广泛的应用前景,已受到越来越多的重视和研究。本论文是基于对功能高分子微球的制备,及其在固定酶,传感器及自组装等方面的应用研究,主要研究内容和结论如下:1、采用间歇无皂乳液聚合法制备了P(SAA)PAA核一壳纳米高分子微球,对在其上固定胰岛素进行了研究。发现固定胰岛素量随着微球粒径的增大而减小,固定胰岛素活性随固定胰岛素量的增大而降低。2、利用自组装的方法,将表面琉基化功能高分子微球、金纳米粒子、辣根过氧化物酶依次组装在金电极上,构建了一种新型的第三代过氧化氢生物传感器。结果表明,该生物传感器在没有电荷传递介质存在的情况下,对过氧化氢显示了诗良高的灵敏度。另外,该生物传感器还具有良好的童复性和长期稳定性。3、采用后加功能单体的无皂乳液聚合法制备出了St/ANPA共聚发光纳米微球。然后利用自组装的方法,得到了不同于无机及金属粒子的高分子发光纳米微球的二维网络结构,以及直径一民度可控的一维发光纳米线。4、采用后加功能单体的无皂乳液聚合法制备了水分散性的St/PANDMEA共聚荧光纳米微球,此微球可用作化学与生物传感器,能很好的检测溶液中的有机亲电子试剂及抗生物素蛋白。5、采用分散聚合法制备了P(St/DVB)高分子微球。然后利用化学沉积法在P(St/DVB)高分子微球表面沉积Ni和Au。得到了核为P(St/DVB)高分子微球,壳为Ni和Au粒子的复合微球。6、利用种子乳液聚合、萃取和表面修饰的方法制备了一种新型的不同于普通的多孔高分子微球的表面含有琉基的AAOVB共聚中空多孔高分子微球。然后利用自组装的方法,制得过氧化氢生物传感器。该生物传感器检测极限达到0.5μM,检测的线性范围从1.0μM到8mM。Functional polymer microspheres have been extensively applied in many fields, such as biochemistry, biomedicine, microelectronics industry etc., owing to their small diameter, large surface to volume ratio and surface functionality. There is much recognition and study just due to their extensively applicable foreground. The present thesis was aimed on the preparation of functional polymer microspheres and their application in immobilization of enzyme, sensors, self-assembly etc. The main research works and the conclusions are as follows: 1. P(St/AA)/PAA nanospheres were prepared by batch emulsifier-free emulsion polymerization. Immobilization of trypsin on them was studied. It was found that the amount of immobilized trypsin increased with decrease of diameter of the polymer microspheres and the activity of immobilized enzyme decreased with increase of amount of immobilized enzyme. 2. Thiol-functionalized P(St/AA)/PAA microspheres, gold nanoparticles and HRP were orderly self-assembled on gold electrode to construct a new third-generation biosensor. Results showed that the biosensor displayed an excellent electrocatalytical response to reduction of hydrogen peroxide without the aid of an electron mediator. Moreover, the biosensor exhibited good reproducibility and long-term stability. 3. Fluorescent St/ANPA copolymer microspheres were prepared by emulsifier-free emulsion polymerization in which functional monomers were added afterward. Then by self-assembly technology, two-dimensional fluorescent network and one-dimensional fluorescent nanowire whose diameter and lengtli could be controlled were prepared. The fluorescent network and nanowire were different from the network and nanowire of inorganic particles and metal particles. 4. Fluorescent St/PANDMEA nanospheres were prepared by emulsifier-free emulsion polymerization in which functional monomers were added afterward. It could be use as chemical and biologic sensor. The sensor exhibited high sensitivity for organic electrophiles and avidin. 5. P(St/DVB) polymer microspheres were prepared by dispersion polymerization. Then Ni and Au particles orderly deposited on the polymer microspheres. The composite microsphere was prepared, whose core and shell were P(St/DVB) polymer microsphere , Ni and Au particles respectively.6 Hollow porous tliiol-fanctionalized P(AA/DVB) microsphere was prepared by seed emulsion polymerization, extraction and surface reaction. It was different from ordinary porous polymer microspheres. Then hydrogen peroxide biosensor was prepared by self-assembly. The detection limit of the biosensor was 0.5μM, and the linear range was from 1.0μM to 8mM

Topics: 无皂乳液聚合, 功能高分子微球, 胰岛素固定, 自组装, 生物传感器, 化学传感器, 分散聚合, 化学沉积, 种子乳液聚合, 高分子化学与物理
Year: 2003
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