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Analyses of PrP<sup>Sc</sup> in the brains of SSBP/1 infected mice.

By Eri Saijo (185915), Hae-Eun Kang (354058), Jifeng Bian (354060), Kristi G. Bowling (478416), Shawn Browning (478417), Sehun Kim (478418), Nora Hunter (209223) and Glenn C. Telling (147325)

Abstract

<p>In <b>A</b> and <b>C</b>, densitometric analysis of immunoblots was used to measure the amounts of protease-resistant OvPrP<sup>Sc</sup> as a function of GdnHCl concentration. The dose-response curve was plotted using a Gaussian non-linear least-square fit. Each point is the mean value derived from densitometric quantification of PK-resistant PrP in three diseased mouse brains. Error bars correspond to standard errors of the mean. <b>A.</b> Conformational stability analysis using mAb 6H4 of OvPrP<sup>Sc</sup>-A136(S) in Tg(OvPrP-A136)3533<sup>+/−</sup> mice (red line), OvPrP<sup>Sc</sup>-V136(U) in Tg(OvPrP-V136)4166<sup>+/−</sup> mice (blue line), and total OvPrP<sup>Sc</sup> in Tg(OvPrP-A/V136) mice (black line). Black asterisks compare differences between OvPrP<sup>Sc</sup>-A136(S) and total PrP<sup>Sc</sup> in Tg(OvPrP-A136)3533<sup>+/−</sup> and Tg(OvPrP-A/V) mice; blue asterisks compare differences between OvPrP<sup>Sc</sup>-A136(S) in Tg(OvPrP-A136)3533<sup>+/−</sup> mice and OvPrP<sup>Sc</sup>-V136(U) in Tg(OvPrP-V136)4166<sup>+/−</sup> mice. <b>C.</b> Conformational stability analysis using mAb PRC5 of OvPrP<sup>Sc</sup>-A136(S) in Tg(OvPrP-A136)3533<sup>+/−</sup> mice (solid line) and OvPrP<sup>Sc</sup>-A136(U) in Tg(OvPrP-A/V136) mice (dashed line). *P<0.05, **P<0.005, ***P<0.001. In <b>B</b> and <b>D</b>, representative immunoblots of PK-resistant PrP in the brains of three mice from each infected cohort of Tg(OvPrP-A136)3533<sup>+/−</sup>, Tg(OvPrP-V136)4166<sup>+/−</sup>, and Tg(OvPrP-A/V136) mice using mAb 6H4 (<b>B</b>) and mAb PRC5 (<b>C</b>). Times of onset of disease for analyzed mice are also provided. OvPrP-V136 is indicated by blue symbols and text; OvPrP-A136 is indicated by red symbols and text.</p

Topics: Earth and Environmental Sciences, Ecology, Biological Sciences, brains, infected
Year: 2013
DOI identifier: 10.1371/journal.ppat.1003692.g002
OAI identifier: oai:figshare.com:article/838483
Provided by: FigShare
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