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NARF6-NCL clones with inducible NCL (WT or 6/S*A) expression.

By Shu Xiao (292300), Elif Caglar (644219), Priscilla Maldonado (644220), Dibash Das (644221), Zaineb Nadeem (644222), Angela Chi (644223), Benjamin Trinité (644224), Xin Li (51274) and Anjana Saxena (644225)

Abstract

<p><b>(A)</b> Western blot of a representative clone that expresses 3xFlag-NCL under Tet-off promoter in NARF6 cells (derived from U2OS). <b>(B)</b> Western blot analyses for cells grown without Dx for the indicated period showing inducible NCL-expression (WT or 6/S*A). Both WT and 6/S*A led to a net increase in p53 protein levels and corresponding p21 protein levels -the downstream target of p53. <b>(C)</b> Plots of p53 and p21 protein levels shown in 2B. The quantification was done by NIH Image J software. Values were first corrected for the β-actin levels and then compared to Ctrl (no exogenous NCL, no Dx day 7) cells. The graph is representative of at least three independent experiments.</p

Topics: Biological Sciences, bh, wt, p 53 transcriptional activity, E 3 ubiquitin ligase, NCL antagonizes Hdm 2, interphase casein kinase 2, Cell Proliferation Nucleolin, CK 2 consensus serine sites, CK 2, expression, Cell proliferation, dna, CK 2 phosphorylation sites, cdk
Year: 2014
DOI identifier: 10.1371/journal.pone.0109858.g002
OAI identifier: oai:figshare.com:article/1204182
Provided by: FigShare
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