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Foxo1 inhibitor AS1842856 accelerates HIV-1 expression.

By Benjamin Trinité (644224), Chi N. Chan (646016), Caroline S. Lee (646017), Saurabh Mahajan (60802), Yang Luo (350920), Mark A. Muesing (350928), Joy M. Folkvord (207887), Michael Pham (646018), Elizabeth Connick (207890) and David N. Levy (118825)

Abstract

<p>AS1842856 (AS) was applied to cells 1 day before and 1 day after infection with single round GFP reporter virus. CD45RO- naïve T cells are shown. <b>A.</b> GFP and CD62L expression in infected naïve CD4+ T cells on the indicated day after infection. Donor A is shown. <b>B.</b> The number of GFP+ cells on the indicated day after infection. Data are average and SD of the two donors. <b>C.</b> Mean fluorescence intensity (MFI) of GFP in the GFP+ cells. Data are average and SD of the two donors. <b>D.</b> Fold increase in the number and the MFI of GFP+ cells, and total GFP fluorescence in the cells calculated as the product of the number of GFP+ cells multiplied by their MFI. Data are average and SD of the two donors. <b>E.</b> Near-full length reverse transcripts on day 2 after infection for one representative cell donor. Data are averages and SD for replicate PCR reactions for each donor. <b>F.</b> HIV-1 fully spliced RNA presented relative to day 1 DMSO condition. Data are averages and SD for replicate PCR reactions for each donor. <b>G.</b> Virus production measured by genomic RNA in culture medium. Data are averages and SD for replicate PCR reactions for each donor. <b>H.</b> CD62L MFI on the indicated day. Data are MFI of GFP+ cells divided by background staining. Data are average and SD of the two donors. <b>I.</b> CD69 MFI on day 7 post infection on naïve and memory resting CD4+ T cells for donor A.</p

Topics: Uncategorised, ccr, CD 4 T cells, Foxo 1 inhibitor AS 1842856, Foxo 1 Activity, FOXO 1, T cell activation, replication, EDG, cancer therapy target, CD 62L, KLF 2 mRNA, CD 62L gene transcription, CD 69 expression, 1p, Partial T cell activation, Foxo 1 interventions, T cell quiescence, 21CIP, il, Memory T cells, CD 4 T Cells HIV
Year: 2014
DOI identifier: 10.1371/journal.pone.0110719.g006
OAI identifier: oai:figshare.com:article/1207568
Provided by: FigShare
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