Calibration curves obtained with FCS.


<p>The molar concentration of (<i>A</i>) mEos2 and (<i>B</i>) mPlum is shown as a function of the integrated cytosolic fluorescence intensity. Counts are given in arbitrary units and refer to the integrated fluorescence intensity of the wide-field image of the cell. Mean and standard deviation from three measurements within one cell are shown. A linear fit (solid line) with a 95% confidence interval (dashed lines) is shown. The extrapolated linear fit of the fluorophore concentration as a function of the integrated fluorescence intensity was used for the determination of protein expression levels.</p

Similar works

Full text



Last time updated on 12/02/2018

This paper was published in FigShare.

Having an issue?

Is data on this page outdated, violates copyrights or anything else? Report the problem now and we will take corresponding actions after reviewing your request.