Calibration curves obtained with FCS.

Abstract

<p>The molar concentration of (<i>A</i>) mEos2 and (<i>B</i>) mPlum is shown as a function of the integrated cytosolic fluorescence intensity. Counts are given in arbitrary units and refer to the integrated fluorescence intensity of the wide-field image of the cell. Mean and standard deviation from three measurements within one cell are shown. A linear fit (solid line) with a 95% confidence interval (dashed lines) is shown. The extrapolated linear fit of the fluorophore concentration as a function of the integrated fluorescence intensity was used for the determination of protein expression levels.</p

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Last time updated on 12/02/2018

This paper was published in FigShare.

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