Decreased infiltration of Th1 cells and CD11b⁺ myeloid cells into spinal cord in ES-DC-treated mice.

Abstract

<p>Spinal cord-infiltrating cells isolated from EAE-induced mice, treated subsequently with ES-DCs (2×10⁶ cells/mice) or untreated, were analyzed by flow cytometry to determine the numbers of infiltrating cells at day 15, 19, and 21 after immunization. (A) The total numbers of infiltrating cell into spinal cord in the two groups are shown (<i>n</i> = 5-7 per group). (B–F) The numbers of CD4⁺ T cells, CD11b⁺ myeloid cells, Th1, Th17, and Treg cells in the spinal cord are shown (<i>n</i> = 5–7 per group). (G) The frequencies of Foxp3⁺, IL-17A⁺, IFN-γ⁺, IFN-γ⁺ IL-17A⁺ (double positive) CD4⁺ T cells within the total CD4⁺ T cells and CD11b⁺ myeloid cells in the spinal cord on day 22 after EAE-induction (<i>n</i> = 5–7 per group). Data (mean ± SD) are representative of three independent experiments. *<i>P<</i>0.05, **<i>P</i><0.01, student's <i>t</i> test were used.</p