Effects of mutations in the conserved DAG motif of PVY CP on recognition with MAb1130.

Abstract

<p>The N-terminal 35 residues of CP in PVY<sup>O</sup>-SASA207, PVY<sup>N</sup>-605 and PVY<sup>C</sup>-Adgen are aligned, and the residue D6, which was mutated, is indicated by an arrow. Red highlighting denotes the minimal epitope recognized by MAb1130, and the conserved DAG motif is underlined. Crude proteins extracted from <i>E. coli</i> expressing an individual recombinant PVY<sup>N</sup>-605 CP or mutant were analyzed by SDS-PAGE and stained with Coomassie Blue to detect the proteins. Positions of molecular size markers (in kilodaltons) are shown to the left. Virions of the wild-type viruses PVY<sup>N</sup>-605 and PVY<sup>O</sup>-UK and proteins of <i>E. coli</i> transformed with an empty expression vector were included as positive and negative controls, respectively. DAG, AAG, and NAG indicate the bacterial lysates containing the recombinant CPs of the wild-type PVY<sup>N</sup>-605 and CPs carrying substitution D6A or D6N, respectively (wild-type CP carrying the DAG motif is marked with an asterisk). The corresponding western blot using MAb1130 is shown below the gel.</p

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Last time updated on 12/02/2018

This paper was published in FigShare.

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