AQP2 and p-AQP2 IMCD localization.

Abstract

<p>Immunoperoxidase microscopy of AQP2 and p-AQP2 in the inner medulla. Sections were incubated with affinity-purified anti-AQP2 (<i>A – E</i>) or affinity-purified anti-p-AQP2 antibody (<i>F</i> – <i>J</i>), and labeling was visualized using peroxidase-conjugated secondary antibody. <i>A – B</i>: In standard salt diet Sham rats AQP2 and p-AQP2 labeling is present at the apical and intracellular domains (arrows). <i>C – F</i>: AQP2 and p-AQP2 stainings from L-Sham and L-Sham+d rats were mainly situated in apical domains with weak intracellular labeling. <i>G – L</i>: In contrast, all HF groups demonstrated strong apical immunoperoxidase labeling of AQP2 and p-AQP2 with virtually no staining in intracellular domains. Magnification x 630.</p