Formation of ntES cell colonies derived from the blastocyst embryos of the 3× cloned embryos.
<p>(a) A 3× blastocyst embryo (day 7) and (b) embryonal outgrowths with clearly ICM cells (red arrow) after culture for 5–8 days. (c) Typical morphology of a putative porcine ntES cell colony after passage. Scale bar = 100 μm.</p
Cell Biology, Genetics, Molecular Biology, Physiology, Biotechnology, Evolutionary Biology, Ecology, Developmental Biology, Cancer, Hematology, Primary colony numbers, ES cell lines, ES cell culture system, KSR, TSA, EB, embryo, survival rates, Putative Porcine Embryonic Stem Cell Lines Derived, porcine ntES cells, DMEM, FBS, MEF, MEM, STO, ntES cell lines, Oocyte Bisection Cloning, ntES cells
DOI identifier: 10.1371/journal.pone.0118165.g002
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