Analysis of LgAQP1-GFP expression by Western blot.
<p>Anti-GFP monoclonal antibody was used to confirm the expression of the LgAQP1WT-GFP and LgAQP1G133D-GFP fusion proteins from protein extracts prepared from LgSb<sup>III</sup>650.2 parasites transfected with pSP72αZEOαLgAQP1WT (lane 1), pSP72αZEOαLgAQP1G133D (lane 2) or pSP72αZEOα (lane 3). Anti-α-tubulin antiserum was used as a loading control.</p
Biological Sciences, guyanensis BackgroundAntimony resistance, parasite, Viannia, alteration, Aquaglyceroporin AQP 1 Gene, LgAQP 1, Antimony Resistant Leishmania, G 133D point mutation, antimony accumulation assays, subtelomeric, intrachromosomal gene amplification, mutant, chromosome, deletion, gene coding
DOI identifier: 10.1371/journal.pntd.0003476.g009
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