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Analysis of LgAQP1-GFP expression by Western blot.

By Rubens Monte-Neto (697658), Marie-Claude N. Laffitte (669627), Philippe Leprohon (133682), Priscila Reis (697659), Frédéric Frézard (220018) and Marc Ouellette (51263)

Abstract

<p>Anti-GFP monoclonal antibody was used to confirm the expression of the LgAQP1WT-GFP and LgAQP1G133D-GFP fusion proteins from protein extracts prepared from LgSb<sup>III</sup>650.2 parasites transfected with pSP72αZEOαLgAQP1WT (lane 1), pSP72αZEOαLgAQP1G133D (lane 2) or pSP72αZEOα (lane 3). Anti-α-tubulin antiserum was used as a loading control.</p

Topics: Biological Sciences, guyanensis BackgroundAntimony resistance, parasite, Viannia, alteration, Aquaglyceroporin AQP 1 Gene, LgAQP 1, Antimony Resistant Leishmania, G 133D point mutation, antimony accumulation assays, subtelomeric, intrachromosomal gene amplification, mutant, chromosome, deletion, gene coding
Year: 2015
DOI identifier: 10.1371/journal.pntd.0003476.g009
OAI identifier: oai:figshare.com:article/1322989
Provided by: FigShare
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