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Sildenafil induces in vitro angiogenesis, DRG neurite outgrowth and Schwann cell migration.

By Lei Wang (6656), Michael Chopp (95019), Alexandra Szalad (692785), LongFei Jia (3150060), XueRong Lu (692786), Mei Lu (177815), Li Zhang (8200), Yi Zhang (9093), RuiLan Zhang (692787) and Zheng Gang Zhang (95014)

Abstract

<p>Representative microscopic images (A to D) and quantitative data (I) show capillary-like tube formation of endothelial cells under regular glucose (RG), high glucose (HG), high glucose with sildenafil (HG+sil, 300 nM), and high glucose with sildenafil in the presence of a neutralizing antibody against Tie2 (+Tie2, 5 μg/ml). Panels E to H show NFH immunoreactive DRG neuron cultured in conditional medium derived from endothelial cells treated with regular glucose (RG), high glucose (HG), high glucose with sildenafil (HG+sil, 300 nM), and high glucose with sildenafil in the presence of a neutralizing antibody against Tie2 (+Tie2, 5 μg/ml). Panels J and K show quantitative data of neurite outgrowth from DRG neurons and the percentage of Schwann cell migration under different conditions listed above. Panel L shows ELISA data of Ang1 levels in conditional medium harvested from endothelial cells cultured with regular glucose (RG), high glucose (HG), high glucose with sildenafil (+sil, 300 nM). *p<0.05 versus regular glucose (RG), and #p<0.05 versus high glucose (HG), respectively. n = 6/group. Bar in D = 50 μm and Bar in H = 20 μm.</p

Topics: Biological Sciences, drg, glucose levels, age 36 weeks, sciatic nerve, Type II Diabetic Mice Diabetic, neurite outgrowth, dorsal root ganglia, mde, Ang 1 receptor, sildenafil, type II diabetes, Sildenafil Ameliorates Long Term Peripheral Neuropathy, neuropathy, phosphodiesterase type 5 enzyme
Year: 2015
DOI identifier: 10.1371/journal.pone.0118134.g004
OAI identifier: oai:figshare.com:article/1311020
Provided by: FigShare
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