Abstract

<p>A: Distinct expression of Dclk1 is associated with ADM. Dclk1 immunostaining in pancreas of C57Bl/6 mice treated with caerulein on day 0 (control), 1, 3, and 5 post injection. B: Dclk1 protein levels are upregulated in the serum of pre-cancerous and cancerous KPC mice. The serum of 5-, 15- (pre-cancerous) and 25- (cancerous) week old control (con) and KPC mice (n = 5 for each group) was purified and subjected to SDS-PAGE and immuno-blotted with anti-Dclk1 antibody. Dclk1 levels are upregulated in serum of pre-cancerous and cancerous KPC mice relative to controls (<i>p</i><0.01). C: Dclk1 is expressed on the surface of circulating tumor cells in KPCY mice. Whole blood was collected from 4-month old KPCY mice (n = 3), incubated with anti-Dclk1 antibody conjugated with AlexaFluor-546, and subjected to flow cytometry. Among the YFP+ circulating cells, 52% were positive for Dclk1. D: Dclk1 is co-localized with YFP in both primary and metastatic tumors. Immuno-staining demonstrating both primary PDAC tissue and metastatic PDAC tissue (colon) of KPCY mice express Dclk1 (red) co-localized with YFP+ tumor cells (green). Dclk1/YFP double positive cells appear yellow-orange.</p

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Last time updated on 12/02/2018

This paper was published in FigShare.

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