<p>A: Distinct expression of Dclk1 is associated with ADM. Dclk1 immunostaining in pancreas of C57Bl/6 mice treated with caerulein on day 0 (control), 1, 3, and 5 post injection. B: Dclk1 protein levels are upregulated in the serum of pre-cancerous and cancerous KPC mice. The serum of 5-, 15- (pre-cancerous) and 25- (cancerous) week old control (con) and KPC mice (n = 5 for each group) was purified and subjected to SDS-PAGE and immuno-blotted with anti-Dclk1 antibody. Dclk1 levels are upregulated in serum of pre-cancerous and cancerous KPC mice relative to controls (<i>p</i><0.01). C: Dclk1 is expressed on the surface of circulating tumor cells in KPCY mice. Whole blood was collected from 4-month old KPCY mice (n = 3), incubated with anti-Dclk1 antibody conjugated with AlexaFluor-546, and subjected to flow cytometry. Among the YFP+ circulating cells, 52% were positive for Dclk1. D: Dclk1 is co-localized with YFP in both primary and metastatic tumors. Immuno-staining demonstrating both primary PDAC tissue and metastatic PDAC tissue (colon) of KPCY mice express Dclk1 (red) co-localized with YFP+ tumor cells (green). Dclk1/YFP double positive cells appear yellow-orange.</p

Similar works

Full text



Last time updated on 12/02/2018

This paper was published in FigShare.

Having an issue?

Is data on this page outdated, violates copyrights or anything else? Report the problem now and we will take corresponding actions after reviewing your request.