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Proteasome inhibitor inhibits SNARE complex formation.

By Zubair A. Karim (513421), Hari Priya Vemana (513422) and Fadi T. Khasawneh (338378)

Abstract

<p>Human platelets were incubated with proteasome inhibitor (MG132, 10 μM) and stimulated with thrombin (0.025 U/mL, 3 minutes). Platelet lysates were precleared and then incubated with anti-syntaxin-11. Immunoprecipitates were separated by SDS-PAGE and immunoblotted using antibodies to syntaxin-11, SNAP-23, phosho-Ser/Thr/Tyr (pSer/Thr/Tyr), and VAMP-8 as indicated.</p

Topics: Biophysics, Biochemistry, Medicine, Microbiology, Cell Biology, Genetics, Immunology, Cancer, Hematology, Space Science, TAB, TAK, activation, SNARE, platelet function, CARMA, NF, MALT 1 ubiquitination, IKK, MALT 1, proteasome inhibitor blocks CBM
Year: 2015
DOI identifier: 10.1371/journal.pone.0119363.g007
OAI identifier: oai:figshare.com:article/2673424
Provided by: FigShare
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