qPCR-HRM assays quantify alleles within DNA mixtures.
<p>The derivative melt graphs show the T<sub>m</sub> profiles of a representative assay (12) tested with mixtures of clinical samples known to contain only one allele at this assay position. The ratios of genomic DNA from samples containing reference (C) allele to alternate (T) allele in the mixtures were: <b>(A)</b> 1:10, 1:4, 1:2, 1:1 and <b>(B)</b> 10:1, 4:1, 2:1 1:1. The other 41 assays not shown were tested similarly and all performed comparably to detect mixed allelic samples.</p
Biological Sciences, dna, High Resolution Melting, hrm, parasite, snp, barcode, Genotype Plasmodium vivax Infections Plasmodium vivax, Single Nucleotide Polymorphism Barcode, vivax genome sequence data
DOI identifier: 10.1371/journal.pntd.0003539.g001
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