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qPCR-HRM assays quantify alleles within DNA mixtures.

By Mary Lynn Baniecki (703271), Aubrey L. Faust (703272), Stephen F. Schaffner (224042), Daniel J. Park (146372), Kevin Galinsky (703273), Rachel F. Daniels (224050), Elizabeth Hamilton (703274), Marcelo U. Ferreira (138475), Nadira D. Karunaweera (703275), David Serre (2815), Peter A. Zimmerman (137926), Juliana M. Sá (318891), Thomas E. Wellems (318892), Lise Musset (248987), Eric Legrand (248989), Alexandre Melnikov (703276), Daniel E. Neafsey (224044), Sarah K. Volkman (224065), Dyann F. Wirth (224064) and Pardis C. Sabeti (224066)

Abstract

<p>The derivative melt graphs show the T<sub>m</sub> profiles of a representative assay (12) tested with mixtures of clinical samples known to contain only one allele at this assay position. The ratios of genomic DNA from samples containing reference (C) allele to alternate (T) allele in the mixtures were: <b>(A)</b> 1:10, 1:4, 1:2, 1:1 and <b>(B)</b> 10:1, 4:1, 2:1 1:1. The other 41 assays not shown were tested similarly and all performed comparably to detect mixed allelic samples.</p

Topics: Biological Sciences, dna, High Resolution Melting, hrm, parasite, snp, barcode, Genotype Plasmodium vivax Infections Plasmodium vivax, Single Nucleotide Polymorphism Barcode, vivax genome sequence data
Year: 2015
DOI identifier: 10.1371/journal.pntd.0003539.g001
OAI identifier: oai:figshare.com:article/1337977
Provided by: FigShare
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