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MEM inhibits growth and viability of prostate cancer cells.

By Maria Shabbir (709127), Deeba N. Syed (605732), Rahul K. Lall (709128), Muhammad Rashid Khan (536378) and Hasan Mukhtar (410102)

Abstract

<p>a. Prostate cancer cells were treated with MEM for 24/48h, and cell viability was determined by MTT assay. Table shows the IC<sub>50</sub> of <i>CWR</i>22Rν1, C<sub>4-2</sub>, PC-3 and DU145 at 24 and 48h. Mean ± SD of experiments performed in triplicate is shown. <b>b.</b> Dose-dependent effect of MEM on clonogenecity of <i>CWR</i>22Rν1 and C<sub>4-2</sub> cells as detected by colony formation assay. Details are described in material methods. <b>c.</b> Effect of various fractions (n-hexane, ethyl acetate, n-butanol and aqueous) on viability of <i>CWR</i>22R ν1 cells, determined by MTT assay. *p<0.05 and **p<0.01 was considered statistically significant.</p

Topics: Biological Sciences, prostate cancer cells, serum PSA levels reciprocating, prostate cancer cell cultures, prostate cancer model system, Maytenus Royleanus Extract, time course analysis, vivo model, parp, antioxidant activity, caffeic acid, Effective strategies, G 2 phase arrest, intraperitoneal injection, CWR 22R cells, prostate cancer progression, Maytenus royleanus, carbohydrate moieties, cell viability, Prostate cancer, MEM fractions, tumor growth, mtt, ar, cell cycle, cdk inhibitors, Cell death, clonogenic survival assays, apoptotic proteins, stage prostate cancer
Year: 2015
DOI identifier: 10.1371/journal.pone.0119859.g002
OAI identifier: oai:figshare.com:article/1350076
Provided by: FigShare
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