Article thumbnail
Location of Repository

Endogenous EB1 and EB3 co-localize with the ciliary marker centrin-3 in mouse retinal cryosections.

By Juan Hidalgo-de-Quintana (709521), Nele Schwarz (709522), Ingrid P. Meschede (709523), Gabriele Stern-Schneider (709524), Michael B. Powner (362894), Ewan E. Morrison (89387), Clare E. Futter (382884), Uwe Wolfrum (277477), Michael E. Cheetham (179278) and Jacqueline van der Spuy (333600)

Abstract

<p><b>A</b>: Immunohistochemical localization of EB1 (green) and centrin-3 (red) in mouse retinal cryosections. Nuclei are labelled with DAPI (blue). The DIC (differential interference contrast) image on the left illustrates the laminated retinal cytoarchitecture, labelled as follows: ganglion cell layer (GCL), inner plexiform layer (IPL), inner nuclear layer (INL), outer plexiform layer (OPL), outer nuclear layer (ONL), photoreceptor inner segments (IS) and photoreceptor outer segments (OS). <b>B</b>: High magnification of the immunohistochemical localization of EB1 (green) and centrin-3 (red) in photoreceptors. The photoreceptor inner segments (IS) and connecting cilia (cc) are labelled. <b>C</b>: High resolution image of EB1 (green) and centrin-3 (red) in the photoreceptor connecting cilium. The connecting cilium (cc) and basal body (bb) are labelled and an asterisk demarcates the cilia-associated centriole. <b>D</b>: High resolution image of EB3 (red) and centrin-3 (green) in the photoreceptor connecting cilium. The connecting cilium (cc) and basal body (bb) are labelled, and the cilia-associated centriole is demarcated by an asterisk. Scale bars: A, B: 10 μm; C, D: 1 μm.</p

Topics: Biological Sciences, AIPL 1, AIPL 1 harbouring, photoreceptor cells, EB 1, microtubule organising centre, EB proteins, Y 2H analysis, AIPL 1 function, 278X, arpe, lca, microtubule network, Immunofluorescent confocal microscopy, EB 3. EB 1, Leber Congenital Amaurosis Protein AIPL 1, protein aryl hydrocarbon receptor, 197P C 239R, centrosomal marker pericentrin, Photoreceptor Cilium PurposeThe aim
Year: 2015
DOI identifier: 10.1371/journal.pone.0121440.g005
OAI identifier: oai:figshare.com:article/1350823
Provided by: FigShare
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • https://figshare.com/articles/... (external link)
  • Suggested articles


    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.