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Co-localisation of mAb 3G1 and 2G4 staining with flaviviral replication complexes.

By Caitlin A. O’Brien (543089), Jody Hobson-Peters (382818), Alice Wei Yee Yam (382819), Agathe M. G. Colmant (709803), Breeanna J. McLean (709804), Natalie A. Prow (382824), Daniel Watterson (543088), Sonja Hall-Mendelin (300663), David Warrilow (37747), Mah-Lee Ng (709805), Alexander A. Khromykh (555023) and Roy A. Hall (382830)

Abstract

<p>Dual was staining performed on Vero cells infected with WNV<sub>KUNV</sub> MOI: 10 or mock-infected and acetone-fixed at 48 hours post-infection. Merged images showing co-localisation of A) 3G1 (green), and B) 2G4 (green) with flavivirus NS1 labelled with anti-NS1 mAb (4G4, red), flavivirus NS5 labelled with anti-NS5 mAb (5H1, red) and flavivirus NS3 labelled with polyclonal anti-NS3 rabbit sera (red). Nuclei stained with Hoechst nuclear stain (blue). Images were taken at 100x magnification. Scale bar denotes 10 μm.</p

Topics: Biological Sciences, 30 base pairs, mAbs recognise dsRNA, ifa, MAVRIC, mosquito populations, elisa, RNA replication, Viral RNA Intermediates, West Nile virus, novel viruses
Year: 2015
DOI identifier: 10.1371/journal.pntd.0003629.g005
OAI identifier: oai:figshare.com:article/1351386
Provided by: FigShare
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