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Expression of Mf-markers and NF-κB subunits in co-cultures of HSC-3 cells and M1 or R848 Mfs.

By Emma Pirilä (5662789), Otto Väyrynen (712915), Elias Sundquist (712916), Kaisa Päkkilä (712917), Pia Nyberg (114759), Sini Nurmenniemi (114742), Virve Pääkkönen (712918), Paula Pesonen (712919), Dan Dayan (474919), Marilena Vered (474920), Lars Uhlin-Hansen (350451) and Tuula Salo (114754)

Abstract

<p>Vybrant CM-Dil labeled HSC-3 cells (red) and unlabeled M1- or R848 Mfs were incubated with DMSO or 10 ng/ml TNF-α for 30 min where after cells were fixed for immunofluorescence with antibodies for CD68, CD163, NF-κB p50 and p65 subunits. Some samples were pre-incubated with 10 μM BAY 11-7082 prior to TNF-α activation. AlexaFluor488-conjugated secondary antibody was used for visualization. Samples were mounted with DAPI- mountain medium to visualize nuclei (blue). Samples were photographed with a Leica Confocal microscope with 63x oil immersion objective. CD163 and NF-κB p65 immunofluorescence from HSC-3 cell and M1 Mf co-cultures (A). CD68 and NF-κB p50 immunofluorescence from HSC-3 cell and R848 Mf co-cultures (B). Red arrows indicate HSC-3 cells and green arrows Mfs. Scale bars 50 μm.</p

Topics: Biological Sciences, Epidermal growth factor, Human Tongue Squamous Cell Carcinoma Oral tongue, hsc, tam, thp, mouse invasion model, OTSCC patient samples, M 1 Mfs, 3 D tissue invasion model, p 65 subunits, m 2 Mfs, carcinoma cells favouring tumor growth, Macrophages Modulate Migration, cytokine production
Year: 2015
DOI identifier: 10.1371/journal.pone.0120895.g008
OAI identifier: oai:figshare.com:article/1356951
Provided by: FigShare
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