Abstract

<p>(A) Immunofluorescence labelling of cytoskeletons from cells expressing BILBO1:myc, T1:myc, T2:myc, T3:myc and T4:myc using anti-NTD (green) and anti-myc (red) after six hours of induction. (B) Immunofluorescence labelling of cytoskeletons expressing BILBO1:myc, T1:myc, T2:myc, T3:myc and T4:myc using anti-NTD (green) and anti-myc (red) after 24 hours of induction. (C) Western-blot of corresponding cells. 2.10<sup>6</sup> non-induced (-) or induced (+) for six hours from whole cells (WC) or cytoskeletons (CK) were loaded on a 12% SDS-PAGE, transferred and immuno-probed with anti-myc, or anti-tubulin (tubulin is a loading control). WT is the non-transfected parental cell line. (D) Growth curves of the parental cell line (WT, black circles) compared to the cell lines non-induced (NI, red open squares) or induced (I, red closed squares) for the expression of BILBO1, T1, T2, T3, T4 myc-tagged truncations. Scale bars in A and B represent 5μm.</p

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Last time updated on 12/02/2018

This paper was published in FigShare.

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