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Binding measurements in CE.

By Yuewei Zhang (521419), Ziqiang Yu (715497), Fei Jiang (324511), Ping Fu (273326), Junjun Shen (715498), Wenxue Wu (413785) and Jinxiang Li (715499)

Abstract

<p>(a) Electropherogram of A9 binding to the purified H9-HA. The purified HA was detected by a UV detector (280 nm) and had a migration time of 5.4 min. A<sub>1</sub>, A<sub>2</sub>, and A<sub>3</sub> represent the areas of peaks of the complexes (A<sub>1</sub>), the aptamer dissociated from the complex during electrophoretic separation (A<sub>2</sub>) and the free aptamer (A<sub>3</sub>) respectively. (b) CE detection of the aptamer-virus interaction. The purified H9N2 AIV was detected by a UV detector (280 nm) and had a peak at the migration time of 7.4 min. The arrows showed the aptamer-virus complex at the migration time of 9.1 min. LIF detector was used for all FAM labeled aptamers. K<sub>d</sub> values were determined with a previously developed CE-based method (see reference [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0123060#pone.0123060.ref032" target="_blank">32</a>]).</p

Topics: Uncategorised, pcr, H 9N AIV, DNA Aptamers, poultry industry, ELAA, ha, pandemic influenza, future development, mdck, AIV subtypes, Viral Infection, influenza virus, Cells New, Avian Influenza H 9N Virus, binding affinity, H 9N AIV infection, H 9N
Year: 2015
DOI identifier: 10.1371/journal.pone.0123060.g002
OAI identifier: oai:figshare.com:article/1363264
Provided by: FigShare
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