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BRCA2-depleted cells exhibit an additive repair defect by pre-IR.

By Nakako Izumi Nakajima (445540), Yoshihiko Hagiwara (715535), Takahiro Oike (483332), Ryuichi Okayasu (445548), Takeshi Murakami (445547), Takashi Nakano (253277) and Atsushi Shibata (445550)

Abstract

<p>A) Pre-IR treatment increases the fraction of BRCA2-knockdown cells with a DNA repair defect. 1BR hTERT cells treated with siControl or siBRCA2 were irradiated with 0.2 Gy X-rays 48 h after siRNA transfection. At 6 h after pre-IR, cells were irradiated with 2 Gy X-rays (challenge IR), and APH was added immediately after the challenge IR. γH2AX foci in G2 cells (CENPF+) were scored. Representative images of G2 cells are shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0122582#pone.0122582.s002" target="_blank">S2A Fig</a>. Error bars represent the SD of three independent experiments. B) Knockdown efficiency of BRCA2 is shown. Arrowhead indicates BRCA2. C) Representative images of the increase in the proportion of BRCA2-depleted cells with a DSB repair defect following pre-IR. Scale bar represent 10 μm (for all images).</p

Topics: Uncategorised, core NHEJ proteins, DNA damage signals, Homologous Recombination Repair, hr, challenge IR, DSB repair pathways, NHEJ repair kinetics, DNA Double Strand Break End Resection, G 2 phase, usage, DSB end resection, repair pathway, DSB repair pathway choice, rpa
Year: 2015
DOI identifier: 10.1371/journal.pone.0122582.g002
OAI identifier: oai:figshare.com:article/1363330
Provided by: FigShare
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