Heparin effects during oligodendrogenesis and oligodendrocyte maturation in CNP::EGFP mice cultures.


<p>A) The number of NG2<sup>+</sup> cells at the end of the proliferation stage are expressed as a proportion respect to the total number of nuclei analyzed per treatment. More than 300 nuclei were analyzed for each treatment in 4 independent cultures. B, C) The proportion of GFP<sup>+</sup> and MBP<sup>+</sup> cells after differentiation is compared in Heparin (1.25 U/L) treated cultures against culture lacking Heparin. More than 600 nuclei were analyzed for a each treatment. Data analysis in graphs was performed using Student´s t Test. D) Representative images of CNP::EGFP mice derived cutlures demonstrating EGFP<sup>+</sup> (green) and MBP<sup>+</sup> (magenta) cells. Scale bar in D (i) represents 100 μm for all images in D. Each pair of columns in A (with or without Heparin) was analyzed with a Student´s t test. ns = not significant.</p

Similar works

Full text

oaioai:figshare.com:article/1366236Last time updated on 2/12/2018

This paper was published in FigShare.

Having an issue?

Is data on this page outdated, violates copyrights or anything else? Report the problem now and we will take corresponding actions after reviewing your request.