Melatonin prevents MPO heme destruction mediated by self-generated HOCl during steady state catalysis.
<p>Fixed amount of MPO (1 μM) was incubated with fixed amount of Cl<sup>-</sup> (100 mM) and increasing concentration of MLT (12–200 μM), and the reaction mixtures were incrementally received fixed amount of H<sub>2</sub>O<sub>2</sub> (20 μM, total concentration of 180 μM). After reaction completion, the spectra of the reaction mixtures were scanned from 300–700 nm.</p
Biological Sciences, hydrogen peroxide, High Performance, hplc, Hypochlorous acid, H 2O ratio, Compound II, electron pathway, ferrozine assay techniques, MPO heme, hocl, MPO heme protection, heme iron, H 2O electrode, Free Iron Mediated, MPO heme moiety, H 2O, Myeloperoxidase Heme Destruction, MPO elevation, MLT oxidation, electron oxidation, MPO activity, feedback mechanism, MPO inhibitor
DOI identifier: 10.1371/journal.pone.0120737.g002
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