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Melatonin prevents MPO heme destruction mediated by self-generated HOCl during steady state catalysis.

By Faten Shaeib (655485), Sana N. Khan (655486), Iyad Ali (655483), Tohid Najafi (571053), Dhiman Maitra (325621), Ibrahim Abdulhamid (716927), Ghassan M. Saed (338910), Subramaniam Pennathur (122576) and Husam M. Abu-Soud (325627)

Abstract

<p>Fixed amount of MPO (1 μM) was incubated with fixed amount of Cl<sup>-</sup> (100 mM) and increasing concentration of MLT (12–200 μM), and the reaction mixtures were incrementally received fixed amount of H<sub>2</sub>O<sub>2</sub> (20 μM, total concentration of 180 μM). After reaction completion, the spectra of the reaction mixtures were scanned from 300–700 nm.</p

Topics: Biological Sciences, hydrogen peroxide, High Performance, hplc, Hypochlorous acid, H 2O ratio, Compound II, electron pathway, ferrozine assay techniques, MPO heme, hocl, MPO heme protection, heme iron, H 2O electrode, Free Iron Mediated, MPO heme moiety, H 2O, Myeloperoxidase Heme Destruction, MPO elevation, MLT oxidation, electron oxidation, MPO activity, feedback mechanism, MPO inhibitor
Year: 2015
DOI identifier: 10.1371/journal.pone.0120737.g002
OAI identifier: oai:figshare.com:article/1366645
Provided by: FigShare
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