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Subcellular localization and functional analysis by Ala-scanning of PTEN N-terminal region reveals distinct subgroups of PTEN mutations.

By Anabel Gil (497053), Isabel Rodríguez-Escudero (724133), Miriam Stumpf (724134), María Molina (512498), Víctor J. Cid (724135) and Rafael Pulido (724136)


<p>(<b>A</b>) Nuclear/cytoplasmic distribution of PTEN N-terminal mutations, monitored as in <a href="" target="_blank">Fig 2C</a>. Nuclear/cytoplasmic distribution of mutations V9A to E18A is as in [<a href="" target="_blank">18</a>]. (<b>B</b>) Influence of PTEN N-terminal mutations in the <i>in vivo</i> PTEN PIP3 phosphatase activity, assessed in yeast. In the upper panel (bars graph), the PIP3 phosphatase activity of PTEN N-terminal mutations was monitored as in <a href="" target="_blank">Fig 2E</a>. In the middle panel (drop growth), growth was monitored as in <a href="" target="_blank">Fig 2D</a>. The activity of mutations K6A to E18A is as in [<a href="" target="_blank">36</a>]. In the bottom panel, the equivalent expression in the yeast of all PTEN mutations, as assessed by immunoblot using anti-PTEN antibodies, is shown. <b>(C)</b> Scheme summarizing the Ala-scanning functional results and the distinct subgroups of PTEN mutations. PTEN N-terminal amino acid sequence (residues 1–43) is shown, and the NLS (grey) motif is indicated. The functional consequences of each Ala-substitution are indicated with a colour code: green (+ +), no effect; yellow (- +), normal PIP3 phosphatase activity but impaired nuclear accumulation; blue (+-), impaired phosphatase activity but normal nuclear accumulation; red (—), impaired phosphatase activity and nuclear accumulation. For nuclear localization in the background of PTEN 1–375, we consider positive those mutants that showed over 50% of cells with nuclear localization. For <i>in vivo</i> activity on PIP3, we consider positive those mutants that showed under 30% of cells with Akt at the plasma membrane and significantly rescued p110α-induced growth inhibition. The first Met (M) was not mutated, and corresponds to PTEN wild type.</p

Topics: Uncategorised, accumulation properties, PIP 3 phosphatase assays, tumor suppressor PTEN, plasma membrane, nls, Tumor Suppressor Activity, alternative plasma membrane, PTEN subcellular localization, localization signal, Functional Dissection, PIP 3 phosphatase, pbm, reconstituted S, PTEN mutations, PTEN Subcellular Targeting, PTEN tumor suppressor activity, activity patterns, PIP 3 phosphatase activity, subcellular locations, Cell proliferation
Year: 2015
DOI identifier: 10.1371/journal.pone.0119287.g003
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Provided by: FigShare
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