Early depletion of inflammatory monocytes protects PbTg infected mice against arising ECM.

Abstract

<p>(A) Effective depletion of inflammatory monocytes (Ly6C^hiLy6G^-) or neutrophils (Ly6C^intLy6G⁺) in the blood of PbTg-infected C57BL/6 mice upon treatment with anti-Gr1 (middle), anti-Ly6G (right), or anti-CCR2 (far right) monoclonal antibodies, on d+1 post depletion. CD11b⁺ leukocytes from the blood (upper row) were gated (squares) and analysed for the presence of Ly6C⁺Ly6G^- monocytes and Ly6^intLy6G⁺neutrophils (lower row) The data of the lower row correspond to data of the upper row. n = 4 mice per group. Flow cytometric analyses show a representative data plot for each group from 1 of 3 independent experiments. (B-G) Groups of PbTg-infected C57BL/6 mice were injected i.p. with anti-Gr1 mAb (B and C), anti-Ly6G mAb (D and E) or anti-CCR2 mAb (F and G) on the first day of infection (d0) or on days 3 and 5 p.i. (d3+5). Scores of cerebral pathology were determined on day 6 p.i. and bar graphs in B, D and F depict the mean and SEM of ECM score in individual mice (n = 8 mice per group). Statistical analysis was performed using Kruskal-Wallis test and Dunn’s Post test (** <i>p</i><0.01). Survival data shown in C, E and G were analyzed with Mantel-Cox log-rank test. <i>p</i><0.05 was considered significant (* <i>p</i><0.05, ** <i>p</i><0.01, *** <i>p</i><0.001). n = 10 mice per group.</p