Article thumbnail
Location of Repository

Early depletion of inflammatory monocytes protects PbTg infected mice against arising ECM.

By Beatrix Schumak (187189), Katrin Klocke (725536), Janina M. Kuepper (725537), Aindrila Biswas (725538), Andrea Djie-Maletz (725539), Andreas Limmer (187212), Nico van Rooijen (4110211), Matthias Mack (148002), Achim Hoerauf (65649) and Ildiko Rita Dunay (725540)

Abstract

<p>(A) Effective depletion of inflammatory monocytes (Ly6C<sup>hi</sup>Ly6G<sup>-</sup>) or neutrophils (Ly6C<sup>int</sup>Ly6G<sup>+</sup>) in the blood of PbTg-infected C57BL/6 mice upon treatment with anti-Gr1 (middle), anti-Ly6G (right), or anti-CCR2 (far right) monoclonal antibodies, on d+1 post depletion. CD11b<sup>+</sup> leukocytes from the blood (upper row) were gated (squares) and analysed for the presence of Ly6C<sup>+</sup>Ly6G<sup>-</sup> monocytes and Ly6<sup>int</sup>Ly6G<sup>+</sup>neutrophils (lower row) The data of the lower row correspond to data of the upper row. n = 4 mice per group. Flow cytometric analyses show a representative data plot for each group from 1 of 3 independent experiments. (B-G) Groups of PbTg-infected C57BL/6 mice were injected i.p. with anti-Gr1 mAb (B and C), anti-Ly6G mAb (D and E) or anti-CCR2 mAb (F and G) on the first day of infection (d0) or on days 3 and 5 p.i. (d3+5). Scores of cerebral pathology were determined on day 6 p.i. and bar graphs in B, D and F depict the mean and SEM of ECM score in individual mice (n = 8 mice per group). Statistical analysis was performed using Kruskal-Wallis test and Dunn’s Post test (** <i>p</i><0.01). Survival data shown in C, E and G were analyzed with Mantel-Cox log-rank test. <i>p</i><0.05 was considered significant (* <i>p</i><0.05, ** <i>p</i><0.01, *** <i>p</i><0.001). n = 10 mice per group.</p

Topics: Biological Sciences, Th 1 responses, Ly 6C monocytes, neutrophils, ifn, cns, T cell frequencies, vivo Ly 6Chi, ctl, ecm, monocytes attenuated brain inflammation, mab, infection, Experimental Cerebral Malaria Plasmodium berghei ANKA, 6g, C 57BL mice, Ly 6Chi
Year: 2015
DOI identifier: 10.1371/journal.pone.0124080.g002
OAI identifier: oai:figshare.com:article/1383318
Provided by: FigShare
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • https://figshare.com/articles/... (external link)
  • Suggested articles


    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.