<p><sup><i>a</i></sup>The codons for mutated amino acids are underlined, with the point mutations in bold.</p><p><sup><i>b</i></sup>The start and stop codons are underlined and the unique nucleotide tail for subsequent mutant strand-specific PCR is in italics.</p><p>Fw, forward; Re, reverse.</p><p>Primers used for multiple site-directed mutagenesis.</p
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