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Aldehyde Dehydrogenase Gene Superfamily in <i>Populus</i>: Organization and Expression Divergence between Paralogous Gene Pairs

By Feng-Xia Tian (729952), Jian-Lei Zang (729953), Tan Wang (721060), Yu-Li Xie (729954), Jin Zhang (53297) and Jian-Jun Hu (729955)

Abstract

<div><p>Aldehyde dehydrogenases (ALDHs) constitute a superfamily of NAD(P)<sup>+</sup>-dependent enzymes that catalyze the irreversible oxidation of a wide range of reactive aldehydes to their corresponding nontoxic carboxylic acids. ALDHs have been studied in many organisms from bacteria to mammals; however, no systematic analyses incorporating genome organization, gene structure, expression profiles, and <i>cis</i>-acting elements have been conducted in the model tree species <i>Populus trichocarpa</i> thus far. In this study, a comprehensive analysis of the <i>Populus ALDH</i> gene superfamily was performed. A total of 26 <i>Populus ALDH</i> genes were found to be distributed across 12 chromosomes. Genomic organization analysis indicated that purifying selection may have played a pivotal role in the retention and maintenance of <i>PtALDH</i> gene families. The exon-intron organizations of <i>PtALDHs</i> were highly conserved within the same family, suggesting that the members of the same family also may have conserved functionalities. Microarray data and qRT-PCR analysis indicated that most <i>PtALDH</i>s had distinct tissue-specific expression patterns. The specificity of <i>cis</i>-acting elements in the promoter regions of the <i>PtALDHs</i> and the divergence of expression patterns between nine paralogous <i>PtALDH</i> gene pairs suggested that gene duplications may have freed the duplicate genes from the functional constraints. The expression levels of some <i>ALDHs</i> were up- or down-regulated by various abiotic stresses, implying that the products of these genes may be involved in the adaptation of <i>Populus</i> to abiotic stresses. Overall, the data obtained from our investigation contribute to a better understanding of the complexity of the <i>Populus ALDH</i> gene superfamily and provide insights into the function and evolution of <i>ALDH</i> gene families in vascular plants.</p></div

Topics: Biological Sciences, nad, model tree species Populus trichocarpa, abiotic stresses, Aldehyde Dehydrogenase Gene Superfamily, Genomic organization analysis, ALDH gene families, expression, PtALDH gene families, Paralogous Gene Pairs Aldehyde dehydrogenases, Populus ALDH gene superfamily, 26 Populus ALDH genes, paralogous PtALDH gene pairs
Year: 2015
DOI identifier: 10.1371/journal.pone.0124669
OAI identifier: oai:figshare.com:article/1394507
Provided by: FigShare
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