Article thumbnail
Location of Repository

LY294002, wortmannin and rapamycin attenuates flagellin-induced cell proliferation.

By Wenlei Bao (735037), Yanfeng Wang (735038), Yuting Fu (735039), Xiaoyang Jia (735040), Jiaxin Li (441199), Nyamtsengel Vangan (735041), Lili Bao (735042), Huifang Hao (336320) and Zhigang Wang (13556)


<p>Ana-1 cells were pretreated with LY294002 (10 μM), wortmannin (10 nM) and rapamycin (100 nM) for 4 h and then stimulated with 100 ng/ml flagellin for 24 h, and cell proliferation was measured by methyl thiazolyl tetrazolium (MTT) assay. A. Proliferation was inhibited by LY294002 and wortmannin in response to flagellin. B. Proliferation was inhibited by rapamycin in response to flagellin. Experiments were made in triplicate, and data are representative of three separate experiments (mean ± SD). *<i>p</i>< 0.05 compare to untreated controls, # <i>p</i>< 0.05 compared with flagellin group.</p

Topics: Biological Sciences, STAT 3, flagellin, rapamycin, PI 3K phosphorylation, PI 3K pathway, tnf, expression, TLR 5. Inhibition, nf, PI 3K, mTORC 1, ly, cytokine, il, response
Year: 2015
DOI identifier: 10.1371/journal.pone.0125910.g003
OAI identifier:
Provided by: FigShare
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • (external link)
  • Suggested articles

    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.