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FBXO44-Mediated Degradation of RGS2 Protein Uniquely Depends on a Cullin 4B/DDB1 Complex - Fig 3

By Benita Sjögren (738128), Steven Swaney (738129) and Richard R. Neubig (234327)

Abstract

<p><b>A</b>. FLAG-FBXO44 co-immunoprecipitates with RGS2-HA. <b>B</b>. The reciprocal experiment shows that RGS2-HA co-immunoprecipitates with FLAG-FBXO44. Also note that co-expression with FLAG-FBXO44 reduces total protein levels of RGS2-HA. <b>C.</b> RGS4<sup>C2S</sup>-HA does not co-immunoprecipitate FLAG-FBXO44. The RGS4<sup>C2S</sup>-HA mutant was used due to the low protein expression obtained with wild-type RGS4-HA. <b>D.</b> Schematic of full length FBXO44 and FBXO44ΔN. <b>E.</b> RGS2-HA co-immunoprecipitates both full length FBXO44 and FBXO44ΔN. <b>F.</b> RGS2 immunoprecipitates with FBXO44 in lysates from mouse whole heart tissue.</p

Topics: Biological Sciences, cullin 4 B, Skp 1, genomic siRNA screening approach, protein degradation, proteasomal degradation, RGS 2 degradation, mediates RGS 2 protein degradation, E 3 ligase, cell function, CUL 4B DNA damage binding protein 1, DDB, RGS 2 dysregulation, novel E 3 ligase, FBXO, bind RGS 2, RGS 2 Protein Uniquely Depends, G Protein Signaling 2
Year: 2015
DOI identifier: 10.1371/journal.pone.0123581.g003
OAI identifier: oai:figshare.com:article/1412969
Provided by: FigShare
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