ERS inhibits insulin receptor signaling in primary rat hippocampal and cortical neurons.

Abstract

<p>(A–B) Western blot analysis of the effect of 4 h tunicamycin (Tm) treatment on p-IRS-1, IRS-1, p-Akt, and Akt levels in primary rat hippocampal (A) and cortical neurons (B). (C–D) Western blot analysis of the effect of 4 h Tm treatment on p-JNK, JNK, p-IRS-1, IRS-1, p-Akt, and Akt levels in primary rat hippocampal (C) and cortical neurons (D) transfected with IRE1α siRNA. (E–F) Western blot analysis of the effect of 4 h Tm treatment on p-IRS-1, IRS-1, p-Akt, and Akt levels in primary rat hippocampal (E) and cortical neurons (F) transfected with JNK siRNA. Statistical analysis is shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0126384#pone.0126384.s001" target="_blank">S1 Fig</a>. Blots were digitized and bands quantified using an image analysis system.</p