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The most important differences between the cloning experiments performed herein.

By Lukasz Michal Szafron (740177), Anna Balcerak (740178), Ewa Anna Grzybowska (740179), Barbara Pienkowska-Grela (740180), Anna Felisiak-Golabek (740181), Agnieszka Podgorska (740182), Magdalena Kulesza (740183), Natalia Nowak (740184), Pawel Pomorski (223458), Juliusz Wysocki (740185), Tymon Rubel (240598), Agnieszka Dansonka-Mieszkowska (740186), Bozena Konopka (740187), Martyna Lukasik (740188) and Jolanta Kupryjanczyk (740189)

Abstract

<p><sup>1</sup>) PCR products shown in this table were first cloned into the pGEM-T Easy vector and then subcloned into one of two target expression vectors listed above. This approach facilitated the cleavage of DNA inserts with restriction enzymes.</p><p><sup>2</sup>) The pCR3-FL2-CRNDEP plasmid was created by subcloning the CRNDEP insert from the pEGFP-N1_CRNDEP plasmid (without PCR reactions); N/A—not applicable.</p><p>The most important differences between the cloning experiments performed herein.</p

Topics: Biological Sciences, CRNDE gene, Proliferating Tissues CRNDE, rna, HeLa cells, Novel Gene CRNDE Encodes, orf, egfp, stress granules, expression, Endogenous CRNDEP localizes
Year: 2015
DOI identifier: 10.1371/journal.pone.0127475.t002
OAI identifier: oai:figshare.com:article/1417483
Provided by: FigShare
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