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Hey1 redirects PAM fusion proteins.

By Daniela Salat (753987), Anja Winkler (164024), Henning Urlaub (22208) and Manfred Gessler (164045)


<p>(A) Schematic representation of PAM-eGFP fusion constructs. RHD: RCC homology domains; PHR: PAM-Highwire-Rpm1 domain. (B) PAM fusion proteins translocate from the cytoplasm to the nucleus upon Hey1 co-expression. HeLa cells were transfected with eGFP-PAM deletion mutants alone or together with mCherry-Hey1. 24 h after transfection cells were fixed and nuclei were stained with Hoechst33342. (C) Quantification of cytoplasmic and nuclear PAM localization. 80 cells were counted per preparation.</p

Topics: Uncategorised, FBXO 45, pam, mass spectrometry analysis, SKP 1. Expression, bHLH Proteins Interact, FBXO 45 Containing SCF Ubiquitin Ligase Complex, protein, tandem affinity purification approach
Year: 2015
DOI identifier: 10.1371/journal.pone.0130288.g005
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Provided by: FigShare
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