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Analysis of EphA2 protein and RNA expression on GFP-control, MLL-AF9 and BCR-ABL bone marrow and spleen.

By Sara Charmsaz (755989), Kirrilee Beckett (755990), Fiona M. Smith (755991), Claudia Bruedigam (755992), Andrew S. Moore (755993), Fares Al-Ejeh (266674), Steven W. Lane (755994) and Andrew W. Boyd (205724)

Abstract

<p>(<b>A</b>) EphA2 mRNA expression levels relative to 18sRNA in bone marrow of GFP-control, MLL-AF9 and BCR-ABL mice, analyzed per 1000000 18s RNA using RT-PCR. Significantly higher EphA2 expression (P = 0.0267) in the MLL-AF9 bone marrow compared to GFP-control mice. (<b>B</b>) Representative flow cytometric overlay analysis of the EphA2 expression in GFP-control, MLL-AF9 and BCR-ABL bone marrow. (<b>C</b>) Flow cytometric analysis of the EphA2 expression in bone marrow of GFP-control, MLL-AF9 and BCR-ABL mice measured as mean fluorescent intensity, showed significantly higher EphA2 expression on MLL-AF9 bone marrow compared to GFP-control bone marrow (P <0.0001) and compared to BCR-ABL leukemic bone marrow (P = 0.0019) (n = 5 GFP-control, n = 4 MLL-AF9, n = 3 BCR-ABL, 1 biological replicates, 2 technical replicates). (<b>D</b>) Flow cytometric analysis of the EphA2 expression in spleen of GFP-control, MLL-AF9 and BCR-ABL mice measured as mean fluorescent intensity, showed significantly higher EphA2 expression in spleen of the MLL-AF9 mice compared to GFP-control mice (P = 0.0143) and higher expression compared to BCR-ABL leukemic mice (n = 5 GFP-control, n = 4 MLL-AF9, n = 3 BCR-ABL, 1 biological replicates, 2 technical replicates). Each dot corresponds to one individual mouse. The data represent the mean ± SEM. Unpaired <i>t</i> test was performed for statistical analyses.</p

Topics: Biological Sciences, receptor tyrosine kinases, EphA 2, EphA 2 expression, EphA 2 knockout mice, leukemic process, role, leukemia, mll
Year: 2015
DOI identifier: 10.1371/journal.pone.0130692.g004
OAI identifier: oai:figshare.com:article/1451735
Provided by: FigShare
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